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Measuring Metabolic Rates: A Manual for Scientists

John R. B. Lighton

Published in print:
2008
Published Online:
September 2008
ISBN:
9780195310610
eISBN:
9780199871414
Item type:
book
Publisher:
Oxford University Press
DOI:
10.1093/acprof:oso/9780195310610.001.0001
Subject:
Biology, Animal Biology, Biotechnology

The measurement of metabolic rates is important in many areas of science. Examples range from ecology through a broad spectrum of physiological disciplines to biomedical fields such as genetic ... More


Flow-Through Respirometry: The Equations

John R. B. Lighton

in Measuring Metabolic Rates: A Manual for Scientists

Published in print:
2008
Published Online:
September 2008
ISBN:
9780195310610
eISBN:
9780199871414
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/acprof:oso/9780195310610.003.0009
Subject:
Biology, Animal Biology, Biotechnology

This chapter de-mystifies respirometry equations, showing how they can be derived using a simple mental trick: concentrating the analysis on the principal gas that is neither consumed nor produced by ... More


A Brief History of Metabolic Measurement

John R. B. Lighton

in Measuring Metabolic Rates: A Manual for Scientists

Published in print:
2008
Published Online:
September 2008
ISBN:
9780195310610
eISBN:
9780199871414
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/acprof:oso/9780195310610.003.0001
Subject:
Biology, Animal Biology, Biotechnology

This chapter describes the evolution of respirometry from Leonardo da Vinci's musings onwards. The works of Boyle, the brilliant and prophetic Mayow, and the well-intentioned but misguided Priestley ... More


Constant Volume Techniques Using Gas Analysis

John R. B. Lighton

in Measuring Metabolic Rates: A Manual for Scientists

Published in print:
2008
Published Online:
September 2008
ISBN:
9780195310610
eISBN:
9780199871414
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/acprof:oso/9780195310610.003.0004
Subject:
Biology, Animal Biology, Biotechnology

By using modern gas analyzers and variations of constant volume techniques described in Chapter 2, simple and high-throughput measurement of the metabolic rates of organisms ranging in size from ... More


Aquatic Oxygen Analysis

John R. B. Lighton

in Measuring Metabolic Rates: A Manual for Scientists

Published in print:
2008
Published Online:
September 2008
ISBN:
9780195310610
eISBN:
9780199871414
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/acprof:oso/9780195310610.003.0005
Subject:
Biology, Animal Biology, Biotechnology

Measuring oxygen consumption rates in aquatic media is the only practical method for determining the metabolic rates of cell cultures and aquatic organisms. This chapter describes the two principal ... More


Measuring Field Metabolic Rates

John R. B. Lighton

in Measuring Metabolic Rates: A Manual for Scientists

Published in print:
2008
Published Online:
September 2008
ISBN:
9780195310610
eISBN:
9780199871414
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/acprof:oso/9780195310610.003.0007
Subject:
Biology, Animal Biology, Biotechnology

This chapter describes various techniques for measuring metabolic rates of unrestrained organisms in the field. These include stable isotope techniques, which allow the accurate measurement of the ... More


Flow-Through Respirometry: Overview

John R. B. Lighton

in Measuring Metabolic Rates: A Manual for Scientists

Published in print:
2008
Published Online:
September 2008
ISBN:
9780195310610
eISBN:
9780199871414
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/acprof:oso/9780195310610.003.0008
Subject:
Biology, Animal Biology, Biotechnology

This chapter describes the basic theory behind the most widely used method for measuring metabolic rates: flow-through or open-system respirometry. The advantages and disadvantages of the technique ... More


Flow-Through Respirometry Using Incurrent Flow Measurement

John R. B. Lighton

in Measuring Metabolic Rates: A Manual for Scientists

Published in print:
2008
Published Online:
September 2008
ISBN:
9780195310610
eISBN:
9780199871414
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/acprof:oso/9780195310610.003.0010
Subject:
Biology, Animal Biology, Biotechnology

This chapter describes the setup, plumbing, and equations required for applying a respirometry system wherein the flow rate of the air entering the animal chamber is known. Such systems are usually ... More


Flow-Through Respirometry Using Excurrent Flow Measurement

John R. B. Lighton

in Measuring Metabolic Rates: A Manual for Scientists

Published in print:
2008
Published Online:
September 2008
ISBN:
9780195310610
eISBN:
9780199871414
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/acprof:oso/9780195310610.003.0011
Subject:
Biology, Animal Biology, Biotechnology

This chapter describes the setup, the plumbing, and the equations for implementing a respirometry system wherein the flow rate of the air leaving the animal chamber is known. Such systems are usually ... More


Coulometric Respirometry

John R. B. Lighton

in Measuring Metabolic Rates: A Manual for Scientists

Published in print:
2018
Published Online:
February 2019
ISBN:
9780198830399
eISBN:
9780191868672
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/oso/9780198830399.003.0003
Subject:
Biology, Biomathematics / Statistics and Data Analysis / Complexity Studies, Ecology

This chapter describes the theory and practical applications of coulometric respirometry. Coulometric respirometry is probably the most accurate method for measuring oxygen consumption rates. It is ... More


Gas pressure, Volume and Flow Measurement

Patrick Magee and Mark Tooley

in The Physics, Clinical Measurement and Equipment of Anaesthetic Practice for the FRCA

Published in print:
2011
Published Online:
November 2020
ISBN:
9780199595150
eISBN:
9780191918032
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/oso/9780199595150.003.0018
Subject:
Clinical Medicine and Allied Health, Anesthesiology

The physics of pressure, flow and the gas laws have been discussed in Chapter 7 in relation to the behaviour of gas and vapour. This section will focus on the physical ... More


Measuring Field Metabolic Rates

John R. B. Lighton

in Measuring Metabolic Rates: A Manual for Scientists

Published in print:
2018
Published Online:
February 2019
ISBN:
9780198830399
eISBN:
9780191868672
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/oso/9780198830399.003.0007
Subject:
Biology, Biomathematics / Statistics and Data Analysis / Complexity Studies, Ecology

This chapter describes various techniques for measuring metabolic rates of unrestrained organisms in the field. These include stable isotope techniques, which allow the accurate measurement of the ... More


Flow-through Respirometry: The Basics

John R. B. Lighton

in Measuring Metabolic Rates: A Manual for Scientists

Published in print:
2018
Published Online:
February 2019
ISBN:
9780198830399
eISBN:
9780191868672
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/oso/9780198830399.003.0008
Subject:
Biology, Biomathematics / Statistics and Data Analysis / Complexity Studies, Ecology

This chapter describes the basic theory behind the most widely used method for measuring metabolic rates: flow-through or open-system respirometry. The advantages and disadvantages of the technique ... More


Measuring the Fire of Life: A Brief History of Metabolic Measurement

John R. B. Lighton

in Measuring Metabolic Rates: A Manual for Scientists

Published in print:
2018
Published Online:
February 2019
ISBN:
9780198830399
eISBN:
9780191868672
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/oso/9780198830399.003.0001
Subject:
Biology, Biomathematics / Statistics and Data Analysis / Complexity Studies, Ecology

This chapter describes the evolution of respirometry from Leonardo da Vinci’s musings onwards. The works of Boyle, the brilliant and prophetic Mayow, and the well-intentioned but misguided Priestley ... More


Constant Volume Techniques Using Gas Analysis

John R. B. Lighton

in Measuring Metabolic Rates: A Manual for Scientists

Published in print:
2018
Published Online:
February 2019
ISBN:
9780198830399
eISBN:
9780191868672
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/oso/9780198830399.003.0004
Subject:
Biology, Biomathematics / Statistics and Data Analysis / Complexity Studies, Ecology

By using modern gas analyzers and variations of constant volume techniques described in Chapter 2, simple and high-throughput measurement of the metabolic rates of organisms ranging in size from ... More


Aquatic Oxygen Analysis

John R. B. Lighton

in Measuring Metabolic Rates: A Manual for Scientists

Published in print:
2018
Published Online:
February 2019
ISBN:
9780198830399
eISBN:
9780191868672
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/oso/9780198830399.003.0005
Subject:
Biology, Biomathematics / Statistics and Data Analysis / Complexity Studies, Ecology

Measuring oxygen consumption rates in aquatic media is the only practical method for determining the metabolic rates of cell cultures and aquatic organisms. This chapter describes the three principal ... More


Flow-through Respirometry: The Equations

John R. B. Lighton

in Measuring Metabolic Rates: A Manual for Scientists

Published in print:
2018
Published Online:
February 2019
ISBN:
9780198830399
eISBN:
9780191868672
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/oso/9780198830399.003.0009
Subject:
Biology, Biomathematics / Statistics and Data Analysis / Complexity Studies, Ecology

This chapter demystifies respirometry equations, showing how they can be derived using a simple mental trick: focusing the analysis on the principal gas that is neither consumed nor produced by ... More


Flow-through Respirometry: Incurrent Flow Measurement

John R. B. Lighton

in Measuring Metabolic Rates: A Manual for Scientists

Published in print:
2018
Published Online:
February 2019
ISBN:
9780198830399
eISBN:
9780191868672
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/oso/9780198830399.003.0010
Subject:
Biology, Biomathematics / Statistics and Data Analysis / Complexity Studies, Ecology

This chapter describes the setup, plumbing, and equations required for applying a respirometry system wherein the flow rate of the air entering the animal chamber is known. Such systems are usually ... More


Flow-through Respirometry: Excurrent Flow Measurement

John R. B. Lighton

in Measuring Metabolic Rates: A Manual for Scientists

Published in print:
2018
Published Online:
February 2019
ISBN:
9780198830399
eISBN:
9780191868672
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/oso/9780198830399.003.0011
Subject:
Biology, Biomathematics / Statistics and Data Analysis / Complexity Studies, Ecology

This chapter describes the setup, plumbing, and equations for implementing a respirometry system wherein the flow rate of the air leaving the animal chamber is known. Such systems are usually ... More


Room Calorimetry and Mask Respirometry

John R. B. Lighton

in Measuring Metabolic Rates: A Manual for Scientists

Published in print:
2018
Published Online:
February 2019
ISBN:
9780198830399
eISBN:
9780191868672
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/oso/9780198830399.003.0012
Subject:
Biology, Biomathematics / Statistics and Data Analysis / Complexity Studies, Ecology

This chapter describes two approaches to human metabolic measurement: room calorimetry and dilution mode mask (or hood, or canopy) respirometry. For room calorimetry, where the EE of a subject is ... More


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