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Phase contrast, contrast transfer function (CTF) and high‐resolution electron microscopy (HRTEM)

Xiaodong Zou, Sven Hovmöller, and Peter Oleynikov

in Electron Crystallography: Electron Microscopy and Electron Diffraction

Published in print:
2011
Published Online:
January 2012
ISBN:
9780199580200
eISBN:
9780191731211
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/acprof:oso/9780199580200.003.0006
Subject:
Physics, Crystallography: Physics

Phase contrast, the contrast transfer function (CTF), kinematical scattering and the weak-phase object approximation are central concepts for image formation. The physics is described in great detail ... More


Electron Microscopy of Macromolecular Assemblies

Joachim Frank

in Three-Dimensional Electron Microscopy of Macromolecular Assemblies: Visualization of Biological Molecules in Their Native State

Published in print:
2006
Published Online:
April 2010
ISBN:
9780195182187
eISBN:
9780199893416
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/acprof:oso/9780195182187.003.0002
Subject:
Biology, Biochemistry / Molecular Biology

Following an outline of the transmission electron microscope (TEM) and its working principles, this chapter starts with a description of specimen preparation methods for EM imaging, including ... More


WAVE OPTICS

JOHN C. H. SPENCE

in High-Resolution Electron Microscopy

Published in print:
2008
Published Online:
January 2010
ISBN:
9780199552757
eISBN:
9780191708664
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/acprof:oso/9780199552757.003.0003
Subject:
Physics, Atomic, Laser, and Optical Physics

This chapter discusses Fresnel diffraction, the diffraction limit, wave and ray aberrations, strong and weak phase objects, single-atom images, and diffractograms for fault diagnosis and for tuning ... More


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