John R. B. Lighton
- Published in print:
- 2008
- Published Online:
- September 2008
- ISBN:
- 9780195310610
- eISBN:
- 9780199871414
- Item type:
- chapter
- Publisher:
- Oxford University Press
- DOI:
- 10.1093/acprof:oso/9780195310610.003.0005
- Subject:
- Biology, Animal Biology, Biotechnology
Measuring oxygen consumption rates in aquatic media is the only practical method for determining the metabolic rates of cell cultures and aquatic organisms. This chapter describes the two principal ...
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Measuring oxygen consumption rates in aquatic media is the only practical method for determining the metabolic rates of cell cultures and aquatic organisms. This chapter describes the two principal variations of aquatic respirometry — closed and open system (or flow-through) respirometry — together with procedures for calibrating aquatic respirometry systems, acquiring data from them, and analyzing the resulting data. The chapter also describes the operation of the widely used Clark dissolved oxygen electrode, the characteristics of common electrode membrane materials, and necessary routine maintenance. There is coverage of common problems, and trouble-shooting guides are included.Less
Measuring oxygen consumption rates in aquatic media is the only practical method for determining the metabolic rates of cell cultures and aquatic organisms. This chapter describes the two principal variations of aquatic respirometry — closed and open system (or flow-through) respirometry — together with procedures for calibrating aquatic respirometry systems, acquiring data from them, and analyzing the resulting data. The chapter also describes the operation of the widely used Clark dissolved oxygen electrode, the characteristics of common electrode membrane materials, and necessary routine maintenance. There is coverage of common problems, and trouble-shooting guides are included.
Robert O. Hall Jr., Serge Thomas, and Evelyn E. Gaiser
- Published in print:
- 2007
- Published Online:
- September 2007
- ISBN:
- 9780195168662
- eISBN:
- 9780199790128
- Item type:
- chapter
- Publisher:
- Oxford University Press
- DOI:
- 10.1093/acprof:oso/9780195168662.003.0010
- Subject:
- Biology, Ecology
Despite a long history of research, measurements of the metabolism of freshwater ecosystems have undergone many recent methodological improvements that are summarized in this chapter. After ...
More
Despite a long history of research, measurements of the metabolism of freshwater ecosystems have undergone many recent methodological improvements that are summarized in this chapter. After describing some relevant considerations about the dynamics of lake and streams, the recommended methods for primary production measurement are detailed. Whole-ecosystem methods of gas exchange are examined, including problems of reaeration and error estimation. An overview of chamber methods using O2 and 14C is provided, including problems of scaling up. Recent advances in microscale methods including oxygen electrodes and fluorometry are described.Less
Despite a long history of research, measurements of the metabolism of freshwater ecosystems have undergone many recent methodological improvements that are summarized in this chapter. After describing some relevant considerations about the dynamics of lake and streams, the recommended methods for primary production measurement are detailed. Whole-ecosystem methods of gas exchange are examined, including problems of reaeration and error estimation. An overview of chamber methods using O2 and 14C is provided, including problems of scaling up. Recent advances in microscale methods including oxygen electrodes and fluorometry are described.
John R. B. Lighton
- Published in print:
- 2018
- Published Online:
- February 2019
- ISBN:
- 9780198830399
- eISBN:
- 9780191868672
- Item type:
- chapter
- Publisher:
- Oxford University Press
- DOI:
- 10.1093/oso/9780198830399.003.0005
- Subject:
- Biology, Biomathematics / Statistics and Data Analysis / Complexity Studies, Ecology
Measuring oxygen consumption rates in aquatic media is the only practical method for determining the metabolic rates of cell cultures and aquatic organisms. This chapter describes the three principal ...
More
Measuring oxygen consumption rates in aquatic media is the only practical method for determining the metabolic rates of cell cultures and aquatic organisms. This chapter describes the three principal variations of aquatic respirometry—closed and open system (or flow-through) respirometry, and headspace respirometry—together with procedures for calibrating aquatic respirometry systems, acquiring data from them, and analyzing the resulting data. Appendix 2 describes the operation of the widely used Clark dissolved oxygen electrode, the characteristics of common electrode membrane materials, and necessary routine maintenance. Common problems are discussed and trouble-shooting guides are included.Less
Measuring oxygen consumption rates in aquatic media is the only practical method for determining the metabolic rates of cell cultures and aquatic organisms. This chapter describes the three principal variations of aquatic respirometry—closed and open system (or flow-through) respirometry, and headspace respirometry—together with procedures for calibrating aquatic respirometry systems, acquiring data from them, and analyzing the resulting data. Appendix 2 describes the operation of the widely used Clark dissolved oxygen electrode, the characteristics of common electrode membrane materials, and necessary routine maintenance. Common problems are discussed and trouble-shooting guides are included.
