Julian C. Knight
- Published in print:
- 2009
- Published Online:
- September 2009
- ISBN:
- 9780199227693
- eISBN:
- 9780191711015
- Item type:
- chapter
- Publisher:
- Oxford University Press
- DOI:
- 10.1093/acprof:oso/9780199227693.003.0009
- Subject:
- Biology, Evolutionary Biology / Genetics, Disease Ecology / Epidemiology
The extent of single nucleotide polymorphism is reviewed, together with insights gained into the nature of allelic architecture in terms of haplotypes, linkage disequilibrium and recombination. The ...
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The extent of single nucleotide polymorphism is reviewed, together with insights gained into the nature of allelic architecture in terms of haplotypes, linkage disequilibrium and recombination. The utility of SNPs in defining genetic determinants of common disease is discussed including the rationale, results and diverse applications of the International HapMap Project. The recent development and application of genome-wide association studies is reviewed including the Wellcome Trust Case Control Consortium study of seven common diseases. Issues relating to design, analysis and interpretation of such studies are described. A detailed review of age-related macular degeneration and inflammatory bowel disease is presented, two common multifactorial diseases where genome-wide association studies have recently enjoyed considerable success. Research in these diseases illustrates the timeline of different approaches used in defining genetic determinants of common disease and how such analyses can provide novel insights into disease pathogenesis.Less
The extent of single nucleotide polymorphism is reviewed, together with insights gained into the nature of allelic architecture in terms of haplotypes, linkage disequilibrium and recombination. The utility of SNPs in defining genetic determinants of common disease is discussed including the rationale, results and diverse applications of the International HapMap Project. The recent development and application of genome-wide association studies is reviewed including the Wellcome Trust Case Control Consortium study of seven common diseases. Issues relating to design, analysis and interpretation of such studies are described. A detailed review of age-related macular degeneration and inflammatory bowel disease is presented, two common multifactorial diseases where genome-wide association studies have recently enjoyed considerable success. Research in these diseases illustrates the timeline of different approaches used in defining genetic determinants of common disease and how such analyses can provide novel insights into disease pathogenesis.
Duncan C. Thomas
- Published in print:
- 2009
- Published Online:
- May 2010
- ISBN:
- 9780195398441
- eISBN:
- 9780199776023
- Item type:
- chapter
- Publisher:
- Oxford University Press
- DOI:
- 10.1093/acprof:oso/9780195398441.003.0008
- Subject:
- Public Health and Epidemiology, Public Health, Epidemiology
The recent availability of commercial high-density genotyping technologies, combined with the identification of subsets of single nucleotide polymorphisms (SNPs) that are capable of “tagging” most of ...
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The recent availability of commercial high-density genotyping technologies, combined with the identification of subsets of single nucleotide polymorphisms (SNPs) that are capable of “tagging” most of the common variants in the human genome from the HapMap project, has now made it feasible to conduct genome-wide association studies (GWAS). There have now been quite a few reviews of the general principles of the design and analysis of GWAS. This chapter focuses on some of the basic issues of multistage sampling design as they have been developed for this purpose, and some of the associated analysis issues.Less
The recent availability of commercial high-density genotyping technologies, combined with the identification of subsets of single nucleotide polymorphisms (SNPs) that are capable of “tagging” most of the common variants in the human genome from the HapMap project, has now made it feasible to conduct genome-wide association studies (GWAS). There have now been quite a few reviews of the general principles of the design and analysis of GWAS. This chapter focuses on some of the basic issues of multistage sampling design as they have been developed for this purpose, and some of the associated analysis issues.
Julian C. Knight
- Published in print:
- 2009
- Published Online:
- September 2009
- ISBN:
- 9780199227693
- eISBN:
- 9780191711015
- Item type:
- chapter
- Publisher:
- Oxford University Press
- DOI:
- 10.1093/acprof:oso/9780199227693.003.0011
- Subject:
- Biology, Evolutionary Biology / Genetics, Disease Ecology / Epidemiology
This chapter discusses the role of genetic variation in modulating gene expression and how this can help resolve functionally important regulatory variants. The successful application of genetic ...
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This chapter discusses the role of genetic variation in modulating gene expression and how this can help resolve functionally important regulatory variants. The successful application of genetic mapping techniques to define expression quantitative trait loci in model organisms including yeast and mice is discussed, together with evidence from studies of human populations. The need to take into account transcript isoform diversity resulting from alternative splicing is highlighted, together with the value of analysis based on allele-specific gene expression and at the protein level. The synergy between genome-wide disease association studies and analysis of the genetics of gene expression, also at a genome-wide level in terms of markers and expression traits, is illustrated by review of recent studies in asthma. The context specificity of regulatory variants is demonstrated, noting the importance of analysis in primary cells or tissues in conditions relevant to the disease or other trait of interest.Less
This chapter discusses the role of genetic variation in modulating gene expression and how this can help resolve functionally important regulatory variants. The successful application of genetic mapping techniques to define expression quantitative trait loci in model organisms including yeast and mice is discussed, together with evidence from studies of human populations. The need to take into account transcript isoform diversity resulting from alternative splicing is highlighted, together with the value of analysis based on allele-specific gene expression and at the protein level. The synergy between genome-wide disease association studies and analysis of the genetics of gene expression, also at a genome-wide level in terms of markers and expression traits, is illustrated by review of recent studies in asthma. The context specificity of regulatory variants is demonstrated, noting the importance of analysis in primary cells or tissues in conditions relevant to the disease or other trait of interest.
Muin J. Khoury, Lars Bertram, Paolo Boffetta, Adam S. Butterworth, Stephen J. Chanock, Siobhan M. Dolan, Isabel Fortier, Montserrat Garcia-Closas, Marta Gwinn, Julian P. T. Higgins, A. Cecile J. W. Janssens, James M. Ostell, Ryan P. Owen, Roberta A. Pagon, Timothy R. Rebbeck, Nathaniel Rothman, Jonine L. Bernstein, Paul R. Burton, Harry Campbell, Anand P. Chokkalingam, Helena Furberg, Julian Little, Thomas R. O’Brien, Daniela Seminara, Paolo Vineis, Deborah M. Winn, Wei Yu, and John P. A. Ioannidis
- Published in print:
- 2009
- Published Online:
- May 2010
- ISBN:
- 9780195398441
- eISBN:
- 9780199776023
- Item type:
- chapter
- Publisher:
- Oxford University Press
- DOI:
- 10.1093/acprof:oso/9780195398441.003.0012
- Subject:
- Public Health and Epidemiology, Public Health, Epidemiology
This chapter reports the findings and recommendations from a multidisciplinary workshop, including geneticists, epidemiologists, journal editors, and bioinformatics experts, that was sponsored by the ...
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This chapter reports the findings and recommendations from a multidisciplinary workshop, including geneticists, epidemiologists, journal editors, and bioinformatics experts, that was sponsored by the Human Genome Epidemiology Network (HuGENet) and held in Atlanta on January 24-25, 2008. The meeting was convened in order to discuss synthesis and appraisal of cumulative evidence on genetic associations and to develop a strategy for an online encyclopedia on genetic variation and common human diseases.Less
This chapter reports the findings and recommendations from a multidisciplinary workshop, including geneticists, epidemiologists, journal editors, and bioinformatics experts, that was sponsored by the Human Genome Epidemiology Network (HuGENet) and held in Atlanta on January 24-25, 2008. The meeting was convened in order to discuss synthesis and appraisal of cumulative evidence on genetic associations and to develop a strategy for an online encyclopedia on genetic variation and common human diseases.
Teri Manolio
- Published in print:
- 2009
- Published Online:
- May 2010
- ISBN:
- 9780195398441
- eISBN:
- 9780199776023
- Item type:
- chapter
- Publisher:
- Oxford University Press
- DOI:
- 10.1093/acprof:oso/9780195398441.003.0006
- Subject:
- Public Health and Epidemiology, Public Health, Epidemiology
Environmental modifiers of the effects of genetic variants, or gene-environment interactions, have received increased attention in recent years due to the recognition that genetic variants alone are ...
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Environmental modifiers of the effects of genetic variants, or gene-environment interactions, have received increased attention in recent years due to the recognition that genetic variants alone are unlikely to explain most of the recent increases in chronic diseases. Such increases are more likely due to environmental and behavioral changes interacting with a genetic predisposition, suggesting that failing to identify and control environmental modifiers of disease risk could mask important associations with genetic variants or misestimate the magnitude of their effects. Identifying environmental modifiers of these variants may also be essential in mitigating the risk conferred by these variants. Population-based genetic association studies with detailed characterization of environmental exposures are critical and underused resources for identifying potential interacting factors. This chapter explores the substantial and complementary strengths offered by the two main approaches to these studies — case-control and cohort designs — in the search for the genetic and environmental influences on common diseases.Less
Environmental modifiers of the effects of genetic variants, or gene-environment interactions, have received increased attention in recent years due to the recognition that genetic variants alone are unlikely to explain most of the recent increases in chronic diseases. Such increases are more likely due to environmental and behavioral changes interacting with a genetic predisposition, suggesting that failing to identify and control environmental modifiers of disease risk could mask important associations with genetic variants or misestimate the magnitude of their effects. Identifying environmental modifiers of these variants may also be essential in mitigating the risk conferred by these variants. Population-based genetic association studies with detailed characterization of environmental exposures are critical and underused resources for identifying potential interacting factors. This chapter explores the substantial and complementary strengths offered by the two main approaches to these studies — case-control and cohort designs — in the search for the genetic and environmental influences on common diseases.
Margaret Lock
- Published in print:
- 2013
- Published Online:
- October 2017
- ISBN:
- 9780691149783
- eISBN:
- 9781400848461
- Item type:
- chapter
- Publisher:
- Princeton University Press
- DOI:
- 10.23943/princeton/9780691149783.003.0007
- Subject:
- Anthropology, Social and Cultural Anthropology
This chapter examines findings from the newly developed technology of genome-wide association studies (GWAS) being applied to the investigation of Alzheimer disease (AD), primarily in the United ...
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This chapter examines findings from the newly developed technology of genome-wide association studies (GWAS) being applied to the investigation of Alzheimer disease (AD), primarily in the United States, United Kingdom, and France. These linked research projects make use of many thousands of DNA samples procured from individuals diagnosed with AD, which are then assessed using high-speed throughput technology and compared with control samples, in an attempt to find out what combinations of genes put individuals at increased risk. To date, these enormously expensive projects have provided few if any startling new insights, and many researchers are highly skeptical as to their value. However, others believe that GWAS is a first step toward a more sophisticated way of understanding the interrelated pathways of the numerous genes that appear to be implicated in AD.Less
This chapter examines findings from the newly developed technology of genome-wide association studies (GWAS) being applied to the investigation of Alzheimer disease (AD), primarily in the United States, United Kingdom, and France. These linked research projects make use of many thousands of DNA samples procured from individuals diagnosed with AD, which are then assessed using high-speed throughput technology and compared with control samples, in an attempt to find out what combinations of genes put individuals at increased risk. To date, these enormously expensive projects have provided few if any startling new insights, and many researchers are highly skeptical as to their value. However, others believe that GWAS is a first step toward a more sophisticated way of understanding the interrelated pathways of the numerous genes that appear to be implicated in AD.
Jesus Gonzalez-Bosquet and Stephen J. Chanock
- Published in print:
- 2009
- Published Online:
- May 2010
- ISBN:
- 9780195398441
- eISBN:
- 9780199776023
- Item type:
- chapter
- Publisher:
- Oxford University Press
- DOI:
- 10.1093/acprof:oso/9780195398441.003.0002
- Subject:
- Public Health and Epidemiology, Public Health, Epidemiology
This chapter presents an overview of the development and progress in applications of genomic technologies, with a focus on genomic sequence variation. Topics discussed include genetic variation, ...
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This chapter presents an overview of the development and progress in applications of genomic technologies, with a focus on genomic sequence variation. Topics discussed include genetic variation, genotype analysis, genome-wide association studies, genotyping issues, quality control in the laboratory, and bioinformatics.Less
This chapter presents an overview of the development and progress in applications of genomic technologies, with a focus on genomic sequence variation. Topics discussed include genetic variation, genotype analysis, genome-wide association studies, genotyping issues, quality control in the laboratory, and bioinformatics.
Frank B. Hu
- Published in print:
- 2008
- Published Online:
- September 2009
- ISBN:
- 9780195312911
- eISBN:
- 9780199865260
- Item type:
- chapter
- Publisher:
- Oxford University Press
- DOI:
- 10.1093/acprof:oso/9780195312911.003.0021
- Subject:
- Public Health and Epidemiology, Public Health, Epidemiology
This chapter begins with a review of the genetic factors underlying monogenic and syndromic forms of obesity. It describes the genetics of common obesity, with a particular focus on results from ...
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This chapter begins with a review of the genetic factors underlying monogenic and syndromic forms of obesity. It describes the genetics of common obesity, with a particular focus on results from genome-wide linkage and candidate gene association studies. It also discusses recent findings using the genome-wide association (GWA) approach. Finally, several methodological problems that commonly plague genetic association studies, especially the inability to replicate findings, are addressed.Less
This chapter begins with a review of the genetic factors underlying monogenic and syndromic forms of obesity. It describes the genetics of common obesity, with a particular focus on results from genome-wide linkage and candidate gene association studies. It also discusses recent findings using the genome-wide association (GWA) approach. Finally, several methodological problems that commonly plague genetic association studies, especially the inability to replicate findings, are addressed.
Julian P. T. Higgins and Julian Little
- Published in print:
- 2009
- Published Online:
- May 2010
- ISBN:
- 9780195398441
- eISBN:
- 9780199776023
- Item type:
- chapter
- Publisher:
- Oxford University Press
- DOI:
- 10.1093/acprof:oso/9780195398441.003.0011
- Subject:
- Public Health and Epidemiology, Public Health, Epidemiology
Human Genome Epidemiology (HuGE) reviews have been a cornerstone of the efforts of the Human Genome Epidemiology Network (HuGENet) to develop an online resource to house the cumulative and changing ...
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Human Genome Epidemiology (HuGE) reviews have been a cornerstone of the efforts of the Human Genome Epidemiology Network (HuGENet) to develop an online resource to house the cumulative and changing information on epidemiologic aspects of human genes. HuGE reviews may collate evidence on population frequencies of genetic variants, genotype-phenotype associations, interactions among genes and between genes, and environmental exposures, or a combination of these. More than 70 HuGE reviews have been completed under the auspices of HuGENet, with more than 80 in preparation at the time of writing. This chapter explains what HuGE reviews aim to achieve and describes some key components of the methodology for undertaking them. The material is also directly relevant to reviews and meta-analysis of genetic association studies undertaken by groups outside of HuGENet.Less
Human Genome Epidemiology (HuGE) reviews have been a cornerstone of the efforts of the Human Genome Epidemiology Network (HuGENet) to develop an online resource to house the cumulative and changing information on epidemiologic aspects of human genes. HuGE reviews may collate evidence on population frequencies of genetic variants, genotype-phenotype associations, interactions among genes and between genes, and environmental exposures, or a combination of these. More than 70 HuGE reviews have been completed under the auspices of HuGENet, with more than 80 in preparation at the time of writing. This chapter explains what HuGE reviews aim to achieve and describes some key components of the methodology for undertaking them. The material is also directly relevant to reviews and meta-analysis of genetic association studies undertaken by groups outside of HuGENet.
Muin J. Khoury, Sara R. Bedrosian, Marta Gwinn, Julian Little, Julian P. T. Higgins, and John P. A. Ioannidis
- Published in print:
- 2009
- Published Online:
- May 2010
- ISBN:
- 9780195398441
- eISBN:
- 9780199776023
- Item type:
- chapter
- Publisher:
- Oxford University Press
- DOI:
- 10.1093/acprof:oso/9780195398441.003.0001
- Subject:
- Public Health and Epidemiology, Public Health, Epidemiology
This chapter begins with a discussion of the Rationale for a Second Edition of Human Genome Epidemiology. It then discusses public health applications of genome-wide association studies, the ...
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This chapter begins with a discussion of the Rationale for a Second Edition of Human Genome Epidemiology. It then discusses public health applications of genome-wide association studies, the emergence of public health genomics, and phases of translation research in genomics. An overview of the subsequent chapters is presented.Less
This chapter begins with a discussion of the Rationale for a Second Edition of Human Genome Epidemiology. It then discusses public health applications of genome-wide association studies, the emergence of public health genomics, and phases of translation research in genomics. An overview of the subsequent chapters is presented.
James Tabery
- Published in print:
- 2014
- Published Online:
- January 2015
- ISBN:
- 9780262027373
- eISBN:
- 9780262324144
- Item type:
- chapter
- Publisher:
- The MIT Press
- DOI:
- 10.7551/mitpress/9780262027373.003.0004
- Subject:
- History, History of Science, Technology, and Medicine
In 2003, Terrie Moffitt and Avshalom Caspi published a groundbreaking study examining how the serotonin transporter gene and stressful life events interact to contribute to the risk of developing ...
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In 2003, Terrie Moffitt and Avshalom Caspi published a groundbreaking study examining how the serotonin transporter gene and stressful life events interact to contribute to the risk of developing depression. When dozens of research teams around the globe attempted to replicate that original result, a peculiar thing emerged—some of the studies supported the original finding, but many came back negative. Faced with this dilemma, scientists performed meta-analyses of the replications; however, the meta-analyses only created their own puzzle—one came back supportive of the original finding, while several came back in conflict with it. Scientists studying the nature and nurture of depression were thus unable to agree whether the original study held up to the scrutiny or fell into disrepute, and unable to agree whether research on gene-environment interaction or research on genome wide association studies was the way forward for human genetics. This episode can be understood as the most recent instantiation of a long-standing dispute about gene-environment interaction. This chapter displays how contemporary scientists debating the nature and nurture of depression have repeated arguments for and against interaction that can be traced back through nearly a century of scientific debate.Less
In 2003, Terrie Moffitt and Avshalom Caspi published a groundbreaking study examining how the serotonin transporter gene and stressful life events interact to contribute to the risk of developing depression. When dozens of research teams around the globe attempted to replicate that original result, a peculiar thing emerged—some of the studies supported the original finding, but many came back negative. Faced with this dilemma, scientists performed meta-analyses of the replications; however, the meta-analyses only created their own puzzle—one came back supportive of the original finding, while several came back in conflict with it. Scientists studying the nature and nurture of depression were thus unable to agree whether the original study held up to the scrutiny or fell into disrepute, and unable to agree whether research on gene-environment interaction or research on genome wide association studies was the way forward for human genetics. This episode can be understood as the most recent instantiation of a long-standing dispute about gene-environment interaction. This chapter displays how contemporary scientists debating the nature and nurture of depression have repeated arguments for and against interaction that can be traced back through nearly a century of scientific debate.
Michael Beenstock
- Published in print:
- 2012
- Published Online:
- August 2013
- ISBN:
- 9780262016926
- eISBN:
- 9780262301381
- Item type:
- chapter
- Publisher:
- The MIT Press
- DOI:
- 10.7551/mitpress/9780262016926.003.0007
- Subject:
- Economics and Finance, Econometrics
This chapter explores the possible contribution of genome-wide association studies (GWAS) to social science. Medical scientists have been looking for the human genome for genes that account for ...
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This chapter explores the possible contribution of genome-wide association studies (GWAS) to social science. Medical scientists have been looking for the human genome for genes that account for diseases believed to have a genetic basis. Socioeconomic survey data containing genetic markers obtained from respondents’ DNA are expected to become available in the near future. GWAS has the potential to identify genes associated with schooling, earnings, criminality, marital stability, and a variety of socioeconomic phenomena. However, this chapter argues that GWAS have generally not resulted in major breakthroughs in medicine. The problem is that such studies are based on induction, in contrast to successful science which is based on deduction, and may even be less successful in the social sciences than they are in medicine. The chapter also discusses methodological issues associated with behavioral genetics in the context of the role of heredity in determining human outcomes.Less
This chapter explores the possible contribution of genome-wide association studies (GWAS) to social science. Medical scientists have been looking for the human genome for genes that account for diseases believed to have a genetic basis. Socioeconomic survey data containing genetic markers obtained from respondents’ DNA are expected to become available in the near future. GWAS has the potential to identify genes associated with schooling, earnings, criminality, marital stability, and a variety of socioeconomic phenomena. However, this chapter argues that GWAS have generally not resulted in major breakthroughs in medicine. The problem is that such studies are based on induction, in contrast to successful science which is based on deduction, and may even be less successful in the social sciences than they are in medicine. The chapter also discusses methodological issues associated with behavioral genetics in the context of the role of heredity in determining human outcomes.
Catherine M. Tangen, Marian L. Neuhouser, and Janet L. Stanford
- Published in print:
- 2017
- Published Online:
- December 2017
- ISBN:
- 9780190238667
- eISBN:
- 9780190238698
- Item type:
- chapter
- Publisher:
- Oxford University Press
- DOI:
- 10.1093/oso/9780190238667.003.0053
- Subject:
- Public Health and Epidemiology, Epidemiology, Public Health
Prostate cancer is the most common solid tumor and the second leading cause of cancer-related mortality in American men. Worldwide, prostate cancer ranks second and fifth as a cause of cancer and ...
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Prostate cancer is the most common solid tumor and the second leading cause of cancer-related mortality in American men. Worldwide, prostate cancer ranks second and fifth as a cause of cancer and cancer deaths, respectively. Despite the international burden of disease due to prostate cancer, its etiology is unclear in most cases. Established risk factors include age, race/ancestry, and family history of the disease. Prostate cancer has a strong heritable component, and genome-wide association studies have identified over 110 common risk-associated genetic variants. Family-based sequencing studies have also found rare mutations (e.g., HOXB13) that contribute to prostate cancer susceptibility. Numerous environmental and lifestyle factors (e.g., obesity, diet) have been examined in relation to prostate cancer incidence, but few modifiable exposures have been consistently associated with risk. Some of the variability in results may be related to etiological heterogeneity, with different causes underlying the development of distinct disease subgroups.Less
Prostate cancer is the most common solid tumor and the second leading cause of cancer-related mortality in American men. Worldwide, prostate cancer ranks second and fifth as a cause of cancer and cancer deaths, respectively. Despite the international burden of disease due to prostate cancer, its etiology is unclear in most cases. Established risk factors include age, race/ancestry, and family history of the disease. Prostate cancer has a strong heritable component, and genome-wide association studies have identified over 110 common risk-associated genetic variants. Family-based sequencing studies have also found rare mutations (e.g., HOXB13) that contribute to prostate cancer susceptibility. Numerous environmental and lifestyle factors (e.g., obesity, diet) have been examined in relation to prostate cancer incidence, but few modifiable exposures have been consistently associated with risk. Some of the variability in results may be related to etiological heterogeneity, with different causes underlying the development of distinct disease subgroups.
Christine Sinoquet and Raphaël Mourad
- Published in print:
- 2014
- Published Online:
- December 2014
- ISBN:
- 9780198709022
- eISBN:
- 9780191779619
- Item type:
- chapter
- Publisher:
- Oxford University Press
- DOI:
- 10.1093/acprof:oso/9780198709022.003.0009
- Subject:
- Mathematics, Probability / Statistics, Biostatistics
This chapter offers an in-depth review of recent developments based on probabilistic graphical models (PGMs) and dedicated to two major concerns: the fundamental task of modeling dependences within ...
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This chapter offers an in-depth review of recent developments based on probabilistic graphical models (PGMs) and dedicated to two major concerns: the fundamental task of modeling dependences within genetic data, that is linkage disequilibrium (LD), and the downstream application to genome-wide association studies (GWASs). Throughout the whole chapter, the selected examples illustrate the use of Bayesian networks, as well as that of Markov random fields, including conditional and hidden Markov random fields. First, the chapter surveys PGM-based approaches dedicated to LD modeling. The next section is devoted to PGM-based GWASs and mainly focuses on multilocus approaches, where PGMs allow to fully benefit from LD. This section also provides an illustration for the acknowledgment of confounding factors in GWASs. The next section is dedicated to the detection of epistastic relationships at the genome scale. A recapitulation and a discussion end the chapter. Finally, directions for future works are outlined.Less
This chapter offers an in-depth review of recent developments based on probabilistic graphical models (PGMs) and dedicated to two major concerns: the fundamental task of modeling dependences within genetic data, that is linkage disequilibrium (LD), and the downstream application to genome-wide association studies (GWASs). Throughout the whole chapter, the selected examples illustrate the use of Bayesian networks, as well as that of Markov random fields, including conditional and hidden Markov random fields. First, the chapter surveys PGM-based approaches dedicated to LD modeling. The next section is devoted to PGM-based GWASs and mainly focuses on multilocus approaches, where PGMs allow to fully benefit from LD. This section also provides an illustration for the acknowledgment of confounding factors in GWASs. The next section is dedicated to the detection of epistastic relationships at the genome scale. A recapitulation and a discussion end the chapter. Finally, directions for future works are outlined.
Henrik Jensen, Marta Szulkin, and Jon Slate
- Published in print:
- 2014
- Published Online:
- August 2014
- ISBN:
- 9780199674237
- eISBN:
- 9780191779275
- Item type:
- chapter
- Publisher:
- Oxford University Press
- DOI:
- 10.1093/acprof:oso/9780199674237.003.0013
- Subject:
- Biology, Evolutionary Biology / Genetics, Ecology
Recent development of high-throughput genomics tools has made it possible and affordable to examine the molecular basis of variation in quantitative traits in studies of non-model species in the ...
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Recent development of high-throughput genomics tools has made it possible and affordable to examine the molecular basis of variation in quantitative traits in studies of non-model species in the wild. High-density single nucleotide polymorphism data and genome sequences provide promising methodological advances complementing and strengthening traditional quantitative genetic analyses from long-term pedigrees. This chapter, discusses how high-density genomic data can be used to determine the actual or realised genetic relationship between relatives, which then can be accounted for in further analyses to improve estimates of quantitative genetic parameters, perhaps even without the need to construct a pedigree. Furthermore, this chapter suggests how combining long-term field data with high-density genomic data, to carry out genome-wide association studies or genomic predictions of phenotypes, can provide important insight into the genetic architecture and evolutionary dynamics of fitness-related traits. Empirical results thus far provide good support for the notion that most quantitative genetic traits studied in wild populations have a highly polygenic basis; a key assumption of quantitative genetic analyses. This chapter also discusses how high-density genomic data can be used to identify past signatures of selection in genetic data that can be further compared to loci currently responsible for variation in individual fitness. Finally, this chapter presents some important issues to consider when sampling, storing and preparing DNA for high-throughput genomics analyses. The application of high-throughput genomics tools in quantitative genetic studies of non-model species in the wild shows great promise to increase understanding of ecological and evolutionary processes in natural populations.Less
Recent development of high-throughput genomics tools has made it possible and affordable to examine the molecular basis of variation in quantitative traits in studies of non-model species in the wild. High-density single nucleotide polymorphism data and genome sequences provide promising methodological advances complementing and strengthening traditional quantitative genetic analyses from long-term pedigrees. This chapter, discusses how high-density genomic data can be used to determine the actual or realised genetic relationship between relatives, which then can be accounted for in further analyses to improve estimates of quantitative genetic parameters, perhaps even without the need to construct a pedigree. Furthermore, this chapter suggests how combining long-term field data with high-density genomic data, to carry out genome-wide association studies or genomic predictions of phenotypes, can provide important insight into the genetic architecture and evolutionary dynamics of fitness-related traits. Empirical results thus far provide good support for the notion that most quantitative genetic traits studied in wild populations have a highly polygenic basis; a key assumption of quantitative genetic analyses. This chapter also discusses how high-density genomic data can be used to identify past signatures of selection in genetic data that can be further compared to loci currently responsible for variation in individual fitness. Finally, this chapter presents some important issues to consider when sampling, storing and preparing DNA for high-throughput genomics analyses. The application of high-throughput genomics tools in quantitative genetic studies of non-model species in the wild shows great promise to increase understanding of ecological and evolutionary processes in natural populations.
Christopher Haiman and David J. Hunter
- Published in print:
- 2018
- Published Online:
- February 2018
- ISBN:
- 9780190676827
- eISBN:
- 9780190676858
- Item type:
- chapter
- Publisher:
- Oxford University Press
- DOI:
- 10.1093/oso/9780190676827.003.0004
- Subject:
- Public Health and Epidemiology, Epidemiology, Public Health
This chapter explores the genetic epidemiology of cancer: the identification and quantification of inherited genetic factors, and their potential interaction with the environment, in the etiology of ...
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This chapter explores the genetic epidemiology of cancer: the identification and quantification of inherited genetic factors, and their potential interaction with the environment, in the etiology of cancer in human populations. It also describes the techniques used to identify genetic variants that contribute to cancer susceptibility. It describes the older research methods for identifying the chromosomal localization of high-risk predisposing genes, such as linkage analysis within pedigrees and allele-sharing methods, as it is important to understand the foundations of the field. It also reviews the epidemiologic study designs that can be helpful in identifying low-risk alleles in candidate gene and genome-wide association studies, as well as gene–environment interactions. Finally, it describes some of the genotyping and sequencing platforms commonly employed for high-throughput genome analysis, and the concept of Mendelian randomization and how it may be useful in the study of biomarkers and environmental causes of cancer.Less
This chapter explores the genetic epidemiology of cancer: the identification and quantification of inherited genetic factors, and their potential interaction with the environment, in the etiology of cancer in human populations. It also describes the techniques used to identify genetic variants that contribute to cancer susceptibility. It describes the older research methods for identifying the chromosomal localization of high-risk predisposing genes, such as linkage analysis within pedigrees and allele-sharing methods, as it is important to understand the foundations of the field. It also reviews the epidemiologic study designs that can be helpful in identifying low-risk alleles in candidate gene and genome-wide association studies, as well as gene–environment interactions. Finally, it describes some of the genotyping and sequencing platforms commonly employed for high-throughput genome analysis, and the concept of Mendelian randomization and how it may be useful in the study of biomarkers and environmental causes of cancer.
Meike Bartels and Bart M. L. Baselmans
- Published in print:
- 2015
- Published Online:
- August 2015
- ISBN:
- 9780199686674
- eISBN:
- 9780191766787
- Item type:
- chapter
- Publisher:
- Oxford University Press
- DOI:
- 10.1093/acprof:oso/9780199686674.003.0010
- Subject:
- Psychology, Cognitive Psychology, Evolutionary Psychology
Psychological well-being constitutes a growing area of research in behavioral genetics. Quantitative behavioral genetic studies have revealed that a substantial part (~40%) of the variation in ...
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Psychological well-being constitutes a growing area of research in behavioral genetics. Quantitative behavioral genetic studies have revealed that a substantial part (~40%) of the variation in subjective well-being (SWB) could be attributed to genetic influences, supporting the scientific effort to identify genomic regions associated with well-being. However, this field is still in its infancy and the few attempts are showing mixed results. Therefore, this chapter provides an overview of the existing molecular genetic studies involving psychological well-being. The different methodological approaches, such as linkage and association analysis, are discussed for their strengths and limitations as well as their opportunities. In general, most studies were likely underpowered to detect genetic effects associated with well-being, a phenotype that is expected to be influenced by many genes with small effect sizes. However, a GWAS (>100K) is in the pipeline and will appear in the near future.Less
Psychological well-being constitutes a growing area of research in behavioral genetics. Quantitative behavioral genetic studies have revealed that a substantial part (~40%) of the variation in subjective well-being (SWB) could be attributed to genetic influences, supporting the scientific effort to identify genomic regions associated with well-being. However, this field is still in its infancy and the few attempts are showing mixed results. Therefore, this chapter provides an overview of the existing molecular genetic studies involving psychological well-being. The different methodological approaches, such as linkage and association analysis, are discussed for their strengths and limitations as well as their opportunities. In general, most studies were likely underpowered to detect genetic effects associated with well-being, a phenotype that is expected to be influenced by many genes with small effect sizes. However, a GWAS (>100K) is in the pipeline and will appear in the near future.
Min Chen, Judy Cho, and Hongyu Zhao
- Published in print:
- 2014
- Published Online:
- December 2014
- ISBN:
- 9780198709022
- eISBN:
- 9780191779619
- Item type:
- chapter
- Publisher:
- Oxford University Press
- DOI:
- 10.1093/acprof:oso/9780198709022.003.0012
- Subject:
- Mathematics, Probability / Statistics, Biostatistics
Genome-wide association studies (GWASs) are widely used to identify good candidates of disease-associated genes that are of interest for further follow-up studies. However, knowledge of biological ...
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Genome-wide association studies (GWASs) are widely used to identify good candidates of disease-associated genes that are of interest for further follow-up studies. However, knowledge of biological pathways and interactions may improve the likelihood of making genuine discoveries in GWASs. A number of methods have been developed to incorporate prior biological knowledge when prioritizing genes. However, most methods treat genes in a specific pathway as an exchangeable set without considering the topological structure of the pathway. Based on results obtained from a standard association study on a Crohn’s disease cohort, it is first verified that neighboring genes in a pathway are more likely to share the same disease status. Then, a Markov Random Field (MRF) model is proposed, to incorporate pathway topology for association analysis. We show that the conditional distribution of our MRF model takes on a simple logistic regression form. Finally, we evaluate our model on real data.Less
Genome-wide association studies (GWASs) are widely used to identify good candidates of disease-associated genes that are of interest for further follow-up studies. However, knowledge of biological pathways and interactions may improve the likelihood of making genuine discoveries in GWASs. A number of methods have been developed to incorporate prior biological knowledge when prioritizing genes. However, most methods treat genes in a specific pathway as an exchangeable set without considering the topological structure of the pathway. Based on results obtained from a standard association study on a Crohn’s disease cohort, it is first verified that neighboring genes in a pathway are more likely to share the same disease status. Then, a Markov Random Field (MRF) model is proposed, to incorporate pathway topology for association analysis. We show that the conditional distribution of our MRF model takes on a simple logistic regression form. Finally, we evaluate our model on real data.
Michael Windle (ed.)
- Published in print:
- 2016
- Published Online:
- May 2017
- ISBN:
- 9780262034685
- eISBN:
- 9780262335522
- Item type:
- book
- Publisher:
- The MIT Press
- DOI:
- 10.7551/mitpress/9780262034685.001.0001
- Subject:
- Biology, Biomathematics / Statistics and Data Analysis / Complexity Studies
Findings from the Human Genome Project and from Genome-Wide Association (GWA) studies indicate that many diseases and traits manifest a more complex genomic pattern than previously assumed. These ...
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Findings from the Human Genome Project and from Genome-Wide Association (GWA) studies indicate that many diseases and traits manifest a more complex genomic pattern than previously assumed. These findings, and advances in high-throughput sequencing, suggest that there are many sources of influence—genetic, epigenetic, and environmental. This volume investigates the role of the interactions of genes and environment (G × E) in diseases and traits (referred to by the contributors as complex phenotypes) including depression, diabetes, obesity, and substance use. The contributors first present different statistical approaches or strategies to address G × E and G × G interactions with high-throughput sequenced data, including two-stage procedures to identify G × E and G × G interactions, marker-set approaches to assessing interactions at the gene level, and the use of a partial-least square (PLS) approach. The contributors then turn to specific complex phenotypes, research designs, or combined methods that may advance the study of G × E interactions, considering such topics as randomized clinical trials in obesity research, longitudinal research designs and statistical models, and the development of polygenic scores to investigate G × E interactions. Contributors Fatima Umber Ahmed, Yin-Hsiu Chen, James Y. Dai, Caroline Y. Doyle, Zihuai He, Li Hsu, Shuo Jiao, Erin Loraine Kinnally, Yi-An Ko, Charles Kooperberg, Seunggeun Lee, Arnab Maity, Jeanne M. McCaffery, Bhramar Mukherjee, Sung Kyun Park, Duncan C. Thomas, Alexandre Todorov, Jung-Ying Tzeng, Tao Wang, Michael Windle, Min ZhangLess
Findings from the Human Genome Project and from Genome-Wide Association (GWA) studies indicate that many diseases and traits manifest a more complex genomic pattern than previously assumed. These findings, and advances in high-throughput sequencing, suggest that there are many sources of influence—genetic, epigenetic, and environmental. This volume investigates the role of the interactions of genes and environment (G × E) in diseases and traits (referred to by the contributors as complex phenotypes) including depression, diabetes, obesity, and substance use. The contributors first present different statistical approaches or strategies to address G × E and G × G interactions with high-throughput sequenced data, including two-stage procedures to identify G × E and G × G interactions, marker-set approaches to assessing interactions at the gene level, and the use of a partial-least square (PLS) approach. The contributors then turn to specific complex phenotypes, research designs, or combined methods that may advance the study of G × E interactions, considering such topics as randomized clinical trials in obesity research, longitudinal research designs and statistical models, and the development of polygenic scores to investigate G × E interactions. Contributors Fatima Umber Ahmed, Yin-Hsiu Chen, James Y. Dai, Caroline Y. Doyle, Zihuai He, Li Hsu, Shuo Jiao, Erin Loraine Kinnally, Yi-An Ko, Charles Kooperberg, Seunggeun Lee, Arnab Maity, Jeanne M. McCaffery, Bhramar Mukherjee, Sung Kyun Park, Duncan C. Thomas, Alexandre Todorov, Jung-Ying Tzeng, Tao Wang, Michael Windle, Min Zhang
Tao Wang
- Published in print:
- 2016
- Published Online:
- May 2017
- ISBN:
- 9780262034685
- eISBN:
- 9780262335522
- Item type:
- chapter
- Publisher:
- The MIT Press
- DOI:
- 10.7551/mitpress/9780262034685.003.0005
- Subject:
- Biology, Biomathematics / Statistics and Data Analysis / Complexity Studies
The importance of the gene × gene (G × G) and gene × environment (G × E) interaction has been widely recognized. It is statistically challenging to account for interactions in the analysis of ...
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The importance of the gene × gene (G × G) and gene × environment (G × E) interaction has been widely recognized. It is statistically challenging to account for interactions in the analysis of genome-wide association data. In this chapter, we introduce a gene-based method for modeling G × G and G × E interactions under the regression framework. We evaluate the type 1 error rate and power of this new method by simulations. We apply this method to the endometrial cancer case-control dataset.Less
The importance of the gene × gene (G × G) and gene × environment (G × E) interaction has been widely recognized. It is statistically challenging to account for interactions in the analysis of genome-wide association data. In this chapter, we introduce a gene-based method for modeling G × G and G × E interactions under the regression framework. We evaluate the type 1 error rate and power of this new method by simulations. We apply this method to the endometrial cancer case-control dataset.
