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Experimental resurrection of ancient biomolecules: gene synthesis, heterologous protein expression, and functional assays

Eric A. Gaucher

in Ancestral Sequence Reconstruction

Published in print:
2007
Published Online:
September 2008
ISBN:
9780199299188
eISBN:
9780191714979
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/acprof:oso/9780199299188.003.0014
Subject:
Biology, Evolutionary Biology / Genetics

Approximately twenty studies have emerged where specific molecular systems from extinct organisms have been resurrected for study in the laboratory. These include digestive proteins (ribonucleases, ... More


Dealing with model uncertainty in reconstructing ancestral proteins in the laboratory: examples from archosaur visual pigments and coralfluorescent proteins

Belinda S.W. Chang, Mikhail V. Matz, Steven F. Field, Johannes Müller, and Ilke van Hazel

in Ancestral Sequence Reconstruction

Published in print:
2007
Published Online:
September 2008
ISBN:
9780199299188
eISBN:
9780191714979
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/acprof:oso/9780199299188.003.0015
Subject:
Biology, Evolutionary Biology / Genetics

This chapter discusses efficient experimental strategies to explore variation in ancestral sequence reconstructions. The experimental strategies are illustrated with two examples, ancestral ... More


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