Frederic Lawrence Holmes
- Published in print:
- 2001
- Published Online:
- October 2013
- ISBN:
- 9780300085402
- eISBN:
- 9780300129663
- Item type:
- chapter
- Publisher:
- Yale University Press
- DOI:
- 10.12987/yale/9780300085402.003.0011
- Subject:
- History, History of Science, Technology, and Medicine
This chapter describes Meselson's reaction to the outcome of the transfer experiment begun in Stahl's absence. The outcome, according to Meselson, was ludicrous—an artifact due to his having mixed up ...
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This chapter describes Meselson's reaction to the outcome of the transfer experiment begun in Stahl's absence. The outcome, according to Meselson, was ludicrous—an artifact due to his having mixed up the DNA samples just as Stahl had teasingly said he might. However, the appearance of a “half-heavy band” must be real; there was no way he could have messed things up so as to make that happen. Immediately they made preparations for a second experiment, and Meselson agreed this time to simplify matters by running it only in one direction. They chose to grow the bacteria first on 15N and then switch them to 14N, rather than the other way around, because that strategy minimized the quantity of the expensive isotope required.Less
This chapter describes Meselson's reaction to the outcome of the transfer experiment begun in Stahl's absence. The outcome, according to Meselson, was ludicrous—an artifact due to his having mixed up the DNA samples just as Stahl had teasingly said he might. However, the appearance of a “half-heavy band” must be real; there was no way he could have messed things up so as to make that happen. Immediately they made preparations for a second experiment, and Meselson agreed this time to simplify matters by running it only in one direction. They chose to grow the bacteria first on 15N and then switch them to 14N, rather than the other way around, because that strategy minimized the quantity of the expensive isotope required.
