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Classical cloning, expression, and purification

Jane Skelly, Maninder K. Sohi, and Thil Batuwangala

in Macromolecular Crystallography: conventional and high-throughput methods

Published in print:
2007
Published Online:
September 2007
ISBN:
9780198520979
eISBN:
9780191706295
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/acprof:oso/9780198520979.003.0001
Subject:
Biology, Biochemistry / Molecular Biology

The ideal protein-expression strategy for X-ray structural analysis should provide correctly folded, soluble, and active protein in sufficient quantities for successful crystallization. Subsequent ... More


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