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Macromolecular Crystallography: conventional and high-throughput methods

Mark R. Sanderson and Jane V. Skelly (eds)

Published in print:
2007
Published Online:
September 2007
ISBN:
9780198520979
eISBN:
9780191706295
Item type:
book
Publisher:
Oxford University Press
DOI:
10.1093/acprof:oso/9780198520979.001.0001
Subject:
Biology, Biochemistry / Molecular Biology

Macromolecular crystallography is the study of macromolecules (proteins and nucleic acids) using X-ray crystallographic techniques in order to determine their molecular structure. The knowledge of ... More


Classical cloning, expression, and purification

Jane Skelly, Maninder K. Sohi, and Thil Batuwangala

in Macromolecular Crystallography: conventional and high-throughput methods

Published in print:
2007
Published Online:
September 2007
ISBN:
9780198520979
eISBN:
9780191706295
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/acprof:oso/9780198520979.003.0001
Subject:
Biology, Biochemistry / Molecular Biology

The ideal protein-expression strategy for X-ray structural analysis should provide correctly folded, soluble, and active protein in sufficient quantities for successful crystallization. Subsequent ... More


Automation of non-conventional crystallization techniques for screening and optimization

Naomi E. Chayen

in Macromolecular Crystallography: conventional and high-throughput methods

Published in print:
2007
Published Online:
September 2007
ISBN:
9780198520979
eISBN:
9780191706295
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/acprof:oso/9780198520979.003.0003
Subject:
Biology, Biochemistry / Molecular Biology

The availability of high-quality crystals is crucial to the structure determination of proteins by X-ray diffraction. It is still not understood why some proteins crystallize with ease while others ... More


First analysis of macromolecular crystals

Sherin S. Abdel-Meguid, David Jeruzalmi, and Mark R. Sanderson

in Macromolecular Crystallography: conventional and high-throughput methods

Published in print:
2007
Published Online:
September 2007
ISBN:
9780198520979
eISBN:
9780191706295
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/acprof:oso/9780198520979.003.0004
Subject:
Biology, Biochemistry / Molecular Biology

The past decade and a half have witnessed an almost complete revolution in the way that macromolecular diffraction data are recorded. The promise of diffraction data measurements essentially free of ... More


Molecular replacement techniques for high-throughput structure determination

Marc Delarue

in Macromolecular Crystallography: conventional and high-throughput methods

Published in print:
2007
Published Online:
September 2007
ISBN:
9780198520979
eISBN:
9780191706295
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/acprof:oso/9780198520979.003.0007
Subject:
Biology, Biochemistry / Molecular Biology

In the context of structural genomics projects, there are two main routes to consider for solving efficiently and rapidly the three-dimensional (3D) structure of the target gene products by ... More


Phase refinement through density modification

Jan Pieter Abrahams, Jasper R. Plaisier, Steven Ness, and Navraj S. Pannu

in Macromolecular Crystallography: conventional and high-throughput methods

Published in print:
2007
Published Online:
September 2007
ISBN:
9780198520979
eISBN:
9780191706295
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/acprof:oso/9780198520979.003.0010
Subject:
Biology, Biochemistry / Molecular Biology

This chapter discusses the concept of density modification, where a priori information about the structure of the macromolecule being studied is used to improve phase estimates. These concepts of ... More


Crystallography in the study of protein–DNA interaction

Maninder K. Sohi and Ivan Laponogov

in Macromolecular Crystallography: conventional and high-throughput methods

Published in print:
2007
Published Online:
September 2007
ISBN:
9780198520979
eISBN:
9780191706295
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/acprof:oso/9780198520979.003.0015
Subject:
Biology, Biochemistry / Molecular Biology

In the living cell, protein-DNA interactions take place during genetic processes such as chromatin organization, recombination, replication, transcription, and DNA repair. In order to fully ... More


Introduction

Joachim Frank

in Three-Dimensional Electron Microscopy of Macromolecular Assemblies: Visualization of Biological Molecules in Their Native State

Published in print:
2006
Published Online:
April 2010
ISBN:
9780195182187
eISBN:
9780199893416
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/acprof:oso/9780195182187.003.0001
Subject:
Biology, Biochemistry / Molecular Biology

This introductory chapter begins with an appreciation of the unique position of electron microscopy in biological research as it bridges a wide gap between X-ray crystallography and light microscopy. ... More


On the Diffraction of X-rays by Crystals

Peter B. Moore

in Visualizing the Invisible: Imaging Techniques for the Structural Biologist

Published in print:
2012
Published Online:
May 2015
ISBN:
9780199767090
eISBN:
9780190267841
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/acprof:osobl/9780199767090.003.0004
Subject:
Biology, Biochemistry / Molecular Biology

This chapter introduces the dominance of x-ray crystallography as a technique used in structural biology. X-ray crystallography focuses on the structures of the fundamental molecules in living ... More


Lenses that focus Electrons

Peter B. Moore

in Visualizing the Invisible: Imaging Techniques for the Structural Biologist

Published in print:
2012
Published Online:
May 2015
ISBN:
9780199767090
eISBN:
9780190267841
Item type:
chapter
Publisher:
Oxford University Press
DOI:
10.1093/acprof:osobl/9780199767090.003.0011
Subject:
Biology, Biochemistry / Molecular Biology

This chapter begins by introducing Ernst Ruska and Max Knoll's electron microscope, an invention inspired by modern cathode ray tube (CRT). Microscopy instruments such as transmission electron ... More


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